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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: GABAergic neurons differentiated from BDNF- and Dlx2-modified neural stem cells restore disrupted neural circuits in brainstem stroke

Fig. 6

Overexpression of BDNF and Dlx2 promoted NSCs into GABAergic neurons. A On Day 28 after transplantation, brain sections from BDNF-NSCs (transfected with Lenti-BDNF) and BDNF/Dlx2-NSCs (transfected with Lenti-BDNF/Dlx2) groups were used to detect the phenotypes of GABAergic neurons, glutamatergic neurons, and cholinergic neurons differentiated from NSCs, using anti-GAD65 + 67, anti-NMDAR, and anti-ChAT. GFP + cells expressed synapsin, but PSD95, an excitatory postsynaptic membrane marker, was less expressed. MBP, a marker of myelin sheath, was found around GFP + cells. B Histograms showed the percentages of neurotransmitter markers GAD65 + 67, ChAT, NMDAR-positive cells and of synaptic markers synapsin and PSD 95 in GFP + cells. *P < 0.05, versus the BDNF-NSCs group. N = 6, scale bar = 20 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512