Fig. 7

Grafted NSCs-derived neurons integrated with the host neural circuit. A NSCs transfected with Lenti-BDNF/Dlx2 were transplanted 7 days after brainstem stroke, and 14 days after the transplantation, anterograde trans-monosynaptic tracer AAV2/1-mCherry was injected into the bilateral primary motor cortex (M1), and brain was taken on Day 28. B GFP + mCherry + cells were observed on the contralateral side and ipsilateral side of infarction in the AAV-BDNF/Dlx2-NSCs group (left), but not in the control groups-AAV group (middle) and BDNF/Dlx2-NSCs group (right). N = 6, scale bar = 20 μm. C The schematic diagram of experiment design. D mCherry + GFP- cells represented host cells receiving substances from grafted NSCs. E GFP- mCherry + MAP2 + cells represented host neurons receiving substances from grafted NSCs. N = 6 refers to 6 mice, and each mice had 6 patched cells, scale bar = 20 μm. F GFP + cells (yellow arrow) were recorded by whole-cell patch clamp on Day 28 after transplantation. GFP + cells had spontaneous action potentials (upper right) and fire action potentials (middle right) and displayed spontaneous postsynaptic currents (bottom right). G The electrophysiological parameters of membrane capacitance, input resistance, resting potential, and threshold of GFP + cells were similar to those of host neurons. N = 6 refers to 6 mice, and each mice had 6 patched cells, *P < 0.05