Fig. 5

AT-hMSCs expressed higher levels of CD55 and were less prone to complement injury. CD55 A mRNA and B protein expression by qPCR and flow cytometry analyses. C Flow cytometry analysis of C3 deposition on hMSCs after exposure to serum. Orange area indicates the hMSCs without serum (Control). Black area indicates hMSCs treated with serum and EDTA control. Blue area indicates hMSCs treated with serum. Red area indicated hMSCs treated with mouse serum, following pre-incubation with anti-CD55 antibody. Results are summarised in two graphs showing the D mean %C3 deposition (%hMSCs positive for C3) E mean %C3 deposition following pre-incubation (blocking) with anti-CD55 antibody. F Cytotoxicity assay to demonstrate membrane leakage after exposure to serum, assessed by BCECF, AM dye. The graph shows the % cytotoxicity, measured as % BCECF dye that was released into the supernatant G Linear regression plot of %C3 deposition and %CD55 + cells in hMSCs. Bar graphs show the summary of three independent experiments with mean +/− SEM. *p < 0.05, **p < 0.01, ***p < 0.001