Table 1 Pluripotency marker expression of 2D and 3D expanded cells

Tra-1-60⁻ Tra-1-81⁻

3.31% (IQR 0.95%)

0.00% (IQR 0.00%)

Tra-1-60⁻ Tra-1-81⁺

0.08% (IQR 0.04%)

0.00% (IQR 0.00%)

Tra-1-60⁺ Tra-1-81⁻

11.05% (IQR 2.83%)

1.20% (IQR 0.87%)

Tra-1-60⁺ Tra-1-81⁺

85.57% (IQR 3.75%)

98.80% (IQR 0.87%)

Oct4⁻ Nanog⁻ Sox2⁻

5.47% (IQR 2.21%)

0.09% (IQR 0.09%)

Oct4⁻ Nanog⁻ Sox2⁺

26.92% (IQR 5.98%)

1.13% (IQR 0.42%)

Oct4⁻ Nanog⁺ Sox2⁻

0.09% (IQR 0.04%)

0.01% (IQR 0.01%)

Oct4⁻ Nanog⁺ Sox2⁺

5.89% (IQR 0.85%)

3.95% (IQR 1.30%)

Oct4⁺ Nanog⁻ Sox2⁻

0.27% (IQR 0.16%)

0.01% (IQR 0.01%)

Oct4⁺ Nanog⁻ Sox2⁺

6.46% (IQR 2.19%)

0.25% (IQR 0.08%)

Oct4⁺ Nanog⁺ Sox2⁻

0.32% (IQR 0.15%)

0.00% (IQR 0.00%)

Oct4⁺ Nanog⁺ Sox2⁺

52.45% (IQR 5.61%)

94.25% (IQR 1.40%)