Table 2 Comparison of recent published 3D suspension iPSC expansions studies in different bioreactor geometries
From: Suspension culture improves iPSC expansion and pluripotency phenotype
Reference | Year | iPSC Cell Lines Used | Media | Bioreactor Size and Type | Highest Fold Expansion/Days |
|---|---|---|---|---|---|
Elanzew et al. [67] | 2015 | iLB-C-31f-r1 | mTeSR1, E8 | 50Â mL, Tube rotation (BioLevitator) | Fivefold/4Â days |
Haraguchi et al. [68] | 2015 | 201B7, 253G1 | mTeSR1 | 100Â mL, Horizontal-blade (Integra Biosciences) | Tenfold/12Â days |
Badenes et al. [69] | 2016 | Gibco CD34 + derived | E8 | 50 mL, Horizontal-blade (StemSpan) | 3.5-fold/10 days |
Kropp et al. [26] | 2016 | hCBiPS2, hHSC_F1285T_iPS2 | mTeSR1, E8 | 250Â mL, Eight blade impeller (DASbox) | Sixfold/7Â days |
Meng et al. [70] | 2017 | 4YA, 4YF | mTeSR1 | 100Â mL, Horizontal-blade (NDS) | 12- to 13-fold/5Â days |
Abecasis et al. [71] | 2017 | ChiPS C4, ChiPS C12, ChiPS C15, ChiPS C18, ChiPS C22 | Cellartis DEF-CS Xeno-Free | 200Â mL, Trapezoid-paddle (DASGIP) | 19-fold/4Â days |
Kwok et al. [72] | 2018 | AFiPS, FSiPS | mTeSR1, StemMACs iPS-Brew | 125Â mL, Horizontal-blade (Corning) 1000Â mL, Horizontal-blade (Mobius) | 16-fold/7Â days |
Rodrigues et al. [41] | 2018 | F002.1A.13, Gibco Human Episomal iPSC | E8 | 0.1 L, Vertical- Wheel® (PBS Biotech) 0.5 L, Vertical- Wheel® (PBS Biotech) | 6.7-fold/6 days |
Noguiera et al. [73] | 2019 | F002.1A.13, Gibco Human Episomal iPSC | mTeSR1, mTeSR3D | 0.1 L, Vertical- Wheel® (PBS Biotech) | 9.3-fold/5 days |
Borys et al. [27] | 2020 | 4YA | mTeSR1 | 0.1 L Vertical- Wheel® (PBS Biotech) | 32-fold/6 days |
Borys et al. [20] | 2021 | 4YA | mTeSR1 | 0.1 L, Vertical- Wheel® (PBS Biotech) 0.5 L, Vertical- Wheel® (PBS Biotech) | 32-fold/6 days |
Manstein et al. [74] | 2021 | MHHi006-A, MHHi001-A, MHHi008-A | E8 | 250Â mL, Eight blade impeller (DASbox) | 70-fold/7Â days |
Dang et al. [28] | 2021 | 4YA | mTeSR1, Modified B8 | 0.1 L mL, Vertical- Wheel® (PBS Biotech) 0.5 L, Vertical- Wheel® (PBS Biotech) | 62-fold/7 days |
Current protocol | 2023 | Healthy donor-derived cell lines | StemFlex | 0.1 L mL, Vertical- Wheel® (PBS Biotech) 0.5 L, Vertical- Wheel® (PBS Biotech) | 93.8-fold/5 days |