Table 1 Sequences of PCR primers
Gene name | Â | Sequences (quantitative RT-PCR) |
|---|---|---|
CD90 | Forward | 5ʹ-ATC GCT CTC CTG CTA ACA GTC-3ʹ |
Reverse | 5ʹ-CTC GTA CTG GA TGG GTG AAC T-3ʹ | |
CD29 | Forward | 5ʹ-GTA ACC AAC CGT AGC AAA GGA-3ʹ |
Reverse | 5ʹ-TCC CCT GAT CTT AAT CGC AAA AC-3ʹ | |
CD105 | Forward | 5ʹ-TGC ACT TGG CCT ACA ATT CCA-3ʹ |
Reverse | 5ʹ-AGC TGC CCA CTC AAG GAT CT-3ʹ | |
CD73 | Forward | 5ʹ-CAG ATG TGG GAA GCT CCT GT-3ʹ |
Reverse | 5ʹ-TGA CTG CTG GAA GTG GAG GT-3ʹ | |
CD13 | Forward | 5ʹ-GTG GAC AGC CTG AAG AAC TG-3ʹ |
Reverse | 5ʹ-GGA GCT TTC AGA GAT GCC AG-3ʹ | |
CD34 | Forward | 5ʹ-TGG AAG GTT TGG ATC AGA GC-3ʹ |
Reverse | 5ʹ-ACG GTC CTG CTT ATG GTG AT-3ʹ | |
CD45 | Forward | 5ʹ-CAC GTT AGC ACC CAC TTC AG-3ʹ |
Reverse | 5ʹ-CCG TAG CTG CTT GTA GCC AT-3ʹ | |
CD31 | Forward | 5ʹ-GAA GAC AGG ATG GCT TCG AA-3ʹ |
Reverse | 5ʹ-CCA GCC GTA GTG TCG TTG TA-3ʹ | |
CD11b | Forward | 5ʹ-GCT GGA GGT GGA AAC TTG TC-3ʹ |
Reverse | 5ʹ-TGG TCA CTC TTG GTG GTG TG-3ʹ | |
CD19 | Forward | 5ʹ-GAG AAC CCG GAG ACC TTT GA-3ʹ |
Reverse | 5ʹ-TGC CGA GGT CTG TCT TCT TC-3ʹ | |
FABP4 | Forward | 5′-GCA TGG CCA AAC CTA ACA TGA-3′ |
Reverse | 5′-CCT GGC CCA GTA TGA AGG AAA-3′ | |
PPARγ | Forward | 5′-AGC CTC ATG AAG AGC CTT CCA-3′ |
Reverse | 5′-ACC CTT GCA TCC TTC ACA AGC-3′ | |
Sox9 | Forward | 5ʹ-AGG TGC TCA AAG GCT ACG ACT-3ʹ |
Reverse | 3ʹ-AGA TGT GCG TCT GCT CCG TG-5ʹ | |
Col2A | Forward | 5′-ACT TGC GTC TAC CCC AAT CC–3′ |
Reverse | 5′-ACA GTC TTG CCC CAC TTA CC-3′ | |
Runx2 | Forward | 5′-CCA CGA CAA CCG CAC CAT-3′ |
Reverse | 5′-CAC TCC GGC CCA CAA ATC TC-3′ | |
Osteocalcin | Forward | 5′-CAC TCC TCG CCC TAT TGG C-3′ |
Reverse | 5′-CCC TCC TGC TTG GAC ACA AAG-3′ | |
GAPDH | Forward | 5′-GTG GAT ATT GTT GCC ATC AAT GAC C-3′ |
Reverse | 5′-GCC CCA GCC TTC TTC ATG GTG GT-3′ |