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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Significant improvement of bone marrow-derived MSC expansion from MDS patients by defined xeno-free medium

Fig. 2

Immunophenotypic characterization. A Surface marker expression on cells freshly thawed or expanded over 2 passages with FBS or XF media. The cells are negative for hematopoietic lineage (lin) markers (CD45, CD34, CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a) and positive for mesenchymal markers (CD73, CD90, CD105, CD146 and CD271) B Flow cytometry plots showing the gating strategy with dead cell and lineage exclusion. C Representative histograms of thawed or expanded MSC stained for mesenchymal markers CD73, CD90, CD105, CD146 and CD271 (gray histograms). White histograms showing the respective unstained control

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Stem Cell Research & Therapy

ISSN: 1757-6512