Fig. 4
Impact of culture conditions on gene expression and engraftment. A Volcano plot shows the expression profile of 82 genes expressed after culture with XF compared to FBS media using the Human Mesenchymal Stem Cell RT2 Profiler PCR Array (PAHS-082, SABiosciences, Qiagen, Hilden, Germany). The genes with fold changes higher than the cut-off value (fold change > 2 with p < 0.05) were selected. B Fourteen transcripts (INFG, SOX2, WNT3A, FGF10, JAG1, BDNF, ICAM1, MCAM, KITLG, ITGA6, GDF15, ENG, SLC17A5 and FGF-2) were upregulated during culture in XF media and four transcripts (NES, GDF7, ANPEP and RUNX2) were downregulated. C Relative mRNA expression of the gene LOXL2, a gene our previous data showed upregulation in MDS. D Comparison of the mean hCD45 + cell engraftment from MDS patient material co-transplanted with MSCs expanded with FBS or XF media. There was no difference in human engraftment even for the samples from the same patient and time point (E)