Fig. 7

eMSCs combined with MPA had the highest inhibitory effect on EC growth. A CM obtained from eMSCs combined with MPA had the highest proliferation-inhibiting effect on EC cells. RL95-2 and HEC-1A cells were treated with 10 μM MPA with or without CM of eMSCs for 48 h before Cell Viability Assay. EC organoids were treated with 10 μM MPA with or without CM of eMSCs for 96 h before CCK-8 assay. B CM obtained from eMSCs combined with 20 μM MPA effectively inhibited viability of EC organoids, compared to using CM or MPA alone. Three-dimensional cell viability assay was used to assess the viability of EC organoids treated with MPA and/or CM of eMSCs for 96 h. C DKK1 combined with MPA had highest anti-proliferative effect on EC organoids compared with DKK1 or MPA alone. EC organoids were treated with or without MPA (20 μM) in the presence of DKK1 (100 ng/mL) or not for 96 h to assess cell viability by three-dimensional cell viability assay. NM, normal medium; CM, conditioned medium; Data were analyzed by ratio t-test (A-C). ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001