Fig. 6
The attenuated CM maturation induced by TRPA1 knockdown could be ameliorated by PGC-1α overexpression. A Representative western blots and bar graph showing the expression of PGC-1α in KD control and TRPA1 KD mESC-CMs. B Representative western blots and bar graph showing the expression of PGC-1α in TRPA1 KD mESC-CMs with or without PGC-1α overexpression. C Confocal microscopy images of α-actinin and mitochondria in TRPA1 KD mESC-CMs with or without PGC-1α overexpression. Scale bar = 25 μm. D Bar graphs showing the percentage of cells with disorganized sarcomeres, cell size, and percentage of multi-nucleated cells in TRPA1 KD mESC-CMs with or without PGC-1α overexpression. n = 40–46 cells from 3 independent batches of differentiation. E Bar graphs showing the mitochondrial size (average mitochondrial area and branch length), mitochondrial complexity (number of branches and junctions per mitochondrion), mitochondrial number, and fractional occupancy. n = 21–24 cells from 3 independent batches of differentiation. F, G Representative spontaneous AP tracings of TRPA1 KD mESC-CMs with or without PGC-1α overexpression. H Bar graphs showing the DDR, Vmax-upstroke, MDP, and amplitude of APs. n = 16–17 cells from 3 independent batches of differentiation. Results were expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. Blots presented in panels (A) and (B) have been chopped. Full-length blots are presented in Additional file 3: Fig. S7