Fig. 7
TRPA1 regulated PGC-1α through the p38 MAPK pathway. A Representative western blots and bar graphs showing the expression of p38 MAPK and phospho-p38 MAPK in NRVMs. B Representative western blots and bar graph showing the protein and mRNA expressions of MKP-1 in NRVMs. C Levels of mRNA transcripts encoding TRPA1, ion channels, Ca2+ handling genes, t-tubule formation-related genes, mitochondrial biogenesis dynamics-related genes, and fatty acid β-oxidation-related genes in TRPA1 knockdown cells with or without p38 MAPK inhibitor. Results were expressed as mean ± SEM from 3 independent isolation of NRVM or 3 batches of differentiation. *P < 0.05; **P < 0.01; ***P < 0.001. Blots presented in panels (A) and (B) have been chopped. Full-length blots are presented in Additional file 3: Fig. S7