Fig. 5
From: Gene editing with ‘pencil’ rather than ‘scissors’ in human pluripotent stem cells
Limitation of BEs (A) The existence of multiple substrates in the editing windows causes unintended bystander editing. (B) BEs require PAM sequence (red bases) at proper distance from the target base in editing window (yellow box). The PAM-relaxed BEs (e.g., BEs with NG PAM) to extend the coverage of BEs on target mutations are developed. (C) Typical BEs edit only transition mutations. CGBE enables C:G to G:C base substitution. (D) PE edits transition and transversion point mutations. Created with BioRender.com