Fig. 3

Transplanted human MPCs into mice with a full thickness cartilage defect. Synovial MPCs were injected in NGS mice post-FTCD (A). Cartilage repair across all groups was quantified (B). Safranin O staining of uninjured (C), injured mice injected with saline (F) or MPCs from ACL-I (Inflamed, n = 3 patients) (I), ACL-I (Non-Inflamed, n = 3 patients) (L) or normal (n = 3 three patients) (O) synovium. Col2 (red: D, G, J, M, P) and HNA (blue: E, H, K, N, Q) staining within each group is also presented. Note: The cell lines injected into mice shown in images I and L were derived from the same patient, but different biopsies. Tissue cytometry was employed to quantify the number of Collagen 2 positive cells in the injury site (R) and the HNA positive cells within the injury site and adjacent synovium (S). Scale bar equals 75 µm. n.s. = not significant; *p < 0.05