Fig. 4

Proteomics analysis of human MPC populations from ACL-I joints. Schematic representation of the quantitative shotgun proteomics workflow (A). Distribution of proteins identified in each population (B). Heat map showing pathways regulated by differentially expressed proteins in MPCs from inflamed versus normal synovium generated with Metascape (C). STRING-db analysis of protein–protein interaction networks with elevated proteins in MPCs from inflamed (D) or normal (E) synovium. Colored lines between the proteins indicate different types of interaction evidence: curated databases (teal); experimentally determined interactions (pink); predicted interactions gene neighborhood (green); gene fusions (red); gene co-occurrence (blue); text-mining (yellow); co-expression (black); protein homology (purple). MPC lines derived from n = 3 patients