Fig. 5

Transplanted sub-populations of human MPCs. Synovial MPCs from ACL-I patients (both inflamed (N = 3) and non-inflamed (N = 3)) were purified based on the expression of CD82 and CD59 and injected in NGS mice with a FTCD (A). Cartilage repair across all groups with CD82^HighCD99⁺CD59⁻ or CD82^LowCD99⁺CD59⁺ MPCs were quantified (B). Safranin O staining of mice given CD82^HighCD99⁺CD59⁻ (C,I) or CD82^LowCD99⁺CD59⁺ (F, L) MPCs. Col2 (red: D,G,J,M) and HNA (blue: E, H, K, N) staining within each group is also presented. Note: The cell lines injected in C versus F and I versus L were derived from the same biopsy. Tissue cytometry was employed to quantify the number of Collagen 2 positive cells in the injury site (O) and the HNA positive cells within the injury site and adjacent synovium (P). Scale bar equals 75 µm. n.s. = not significant; *p < 0.05