Fig. 6

Transplanted MPC sub-populations from normal synovium. MPCs from normal synovium were purified based on the expression of CD82 and CD59 and injected in NGS mice with a FTCD (A). Cartilage repair across all groups with CD82^HighCD99⁺CD59⁻ or CD82^LowCD99⁺CD59⁺ MPCs were quantified (B). Safranin O staining of mice given CD82^HighCD99⁺CD59⁻ (C, I) or CD82^LowCD99⁺CD59⁺ (F, L) MPCs. Col2 (red D, G, J, M) and HNA (blue E, H, K, N) staining within each group is also presented. Note: The cell lines injected in C versus F were derived from the same biopsy. Tissue cytometry was employed to quantify the number of Collagen 2 positive cells in the injury site (I) and the HNA positive cells within the injury site and adjacent synovium (J). Scale bar equals 75 µm. n.s. = not significant; *p < 0.05