Fig. 5
From: Cell-free fat extract restores hair loss: a novel therapeutic strategy for androgenetic alopecia
Effect of CEFFE (0, 50, 250, 500 μg/ml; 72 h), DHT (1, 10, 100 μM; 72 h) and DHT + CEFFE (100 μM DHT + 50, 250, 500 μg/ml CEFFE; 72 h) on the intracellular DHT concentration and AR expression of hDPCs. A Quantitative analysis of DHT concentration using ELISA. B RT-qPCR analysis of AR mRNA expression after culturing hDPCs with CEFFE, DHT and DHT + CEFFE, respectively. qRT-PCR analysis showed the expression of AR was increased after DHT treatment but declined after co-incubation with DHT and CEFFE. C Western blot for AR after treating hDPCs with CEFFE, DHT and DHT + CEFFE. Original data was presented in Additional file 2: Fig. 4. It revealed an increase in the expression of AR after incubating with DHT but decreased after co-incubation with DHT and CEFFE. Data represent the mean ± SD; n = 3; *P < 0.05