Fig. 3

Immunofluorescence staining of LMCs demonstrated the expression of diverse surface markers. Immunostaining of LMCs at 24 h after isolation revealed that these cells express CD34 (as a hematopoietic stem cells marker), CD31 (as endothelial cells marker), CD73 (as one of the mesenchymal stem cells markers), and CD68 (as a macrophage lineage marker) at various intensities. The cells were also incubated with isotype control antibodies as negative controls and remained completely unstained (data shown in Additional file 10: Fig. S5). Scale bars: 100 μm. Pie charts show the percentage of positive and negative cells