Fig. 6

Adipocytes as critical elements of tissue regeneration. A Over 28 days post-sub-lethal X-irradiation, the BM sections were inspected and scored for the adipocyte content. The scores are demonstrated in the curve as mean ± SD. Also, a substantial fraction of BM cell pellet constituted of adipocytes during the regenerative process. B Honey-comb structures formed by fat cells. Representative image from H&E section of BM at day16 post-irradiation (left). Culture of ablated BM (day16) after 2 weeks (middle) and 4 weeks (right). C Multinucleated cells were observed around the structures generated by fat cells in the BM sections of the sub-lethally irradiated mice on day 16 post-irradiation. D Adipocytes formed vessel-like structures. Adipocytes lined up immediately after culture (left). Oil Red staining of oriented adipocytes of ablated BM (day 18) 5 weeks after culture (middle). In the BM (day 20), the newly formed vessels contained remnants of adipocytes (left). E Multinucleation of adipocytes. Multinucleated adipocytes were observed by optical microscopy (left) and after Oil Red staining (middle). Another representative is shown in right. Multinucleation of adipocytes was furtherly observed by DAPI staining (lower panels). Representative phenomena are shown by arrowheads