Fig. 8

Transplanted LMCs reconstitute hematopoietic and stromal lineages in myeloablated NOG mice. A Procedure of isolation and transplantation of BM-LMCs. B Flow cytometry of peripheral blood, BM, and spleen for GFP⁺ cells in normal, GFP transgenic, whole BM-transplanted, and LMC-transplanted mice. Dotted circles show the population of GFP-positive cells. C Immunohistochemistry of spleen sections for GFP. Scale bar: 50 μm. D Colonies of GFP⁺ cells two weeks after culturing BM of LMC-transplanted mice. WBM whole bone marrow, LMC large multinucleated cell. Scale bar: 100 μm