Fig. 3
LncRNA00638 is associated with osteogenic differentiation. A The microarray results were validated by RT-qPCR. The RT–qPCR and microarray results were consistent for seven of the selected lncRNAs, among which lncRNA00638 showed the most significant difference, while the difference in TCONS_00008000 was not statistically significant. B Among the seven lncRNAs for which relative expression was detected by RT-qPCR after 7 d of osteogenic induction, lncRNA00638 expression changed most significantly. C Expression levels of lncRNA00638 at 0, 1, 3, 7 and 14 d detected by RT-qPCR. D Pearson correlation analysis showed that the expression level of lncRNA00638 was strongly related to Runx2 (R2 = 0.8935) and Col1 (R2 = 0.8935) during osteogenic induction. Osteo: osteogenic induction. Undiff: no osteogenic induction. **: P < 0.01; *: P < 0.05; NS: no significant difference