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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: LncRNA00638 promotes the osteogenic differentiation of periodontal mesenchymal stem cells from periodontitis patients under static mechanical strain

Fig. 4

LncRNA00638 promotes osteogenic differentiation. A RT-qPCR detection of the infection efficiency of the lncRNA00638-expressing lentivirus. B Expression of Runx2, ALP and Col1 was detected by RT-qPCR after 12% SMS loading and 7 days osteogenic induction. C Images showing ALP and ARS staining. D Quantitative assay of ALP activity. E Quantitative assay of ARS staining. F Expression of Runx2, ALP and Col1 was detected by RT-qPCR after 8% SMS loading and 7 days of osteogenic induction. G Images showing ALP and ARS staining. H Quantitative ALP assay results. I Quantitative ARS staining results. Data are presented as the mean ± SD a: compared with the PPDLSCs group (P < 0.05); b: compared with the PPDLSCs + 12%SMS group (P < 0.05); c: compared with the PPDLSCs + 12% SMS NC group (P < 0.05); d: compared with the PPDLSCs + 12% SMS lncRNA00638 group (P < 0.05); e: compared with the PPDLSCs + 12% SMS lncRNA00638-RNAi (P < 0.05)

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Stem Cell Research & Therapy

ISSN: 1757-6512