Table 1 Primer sequences for osteogenesis-related genes and lncRNAs
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
β-actin | TGGCACCCAGCACAATGA | CTAAGTCATAGTCCGCCTAGAAGCA |
Runx2 | CCCGTGGCCTTCAAGGT | CGTTACCCGCCATGACAGTA |
ALP | GGACCATTCCCACGTCTTCAC | CCTTGTAGCCAGGCCCATTG |
OCN | CCCAGGCGCTACCTGTATCAA | GGTCAGCCAACTCGTCACAGTC |
ENST00000550947 | AAGCTTGGCTGTGTCTGTCTAGTG | GCGGATGCCTTCTCTCTAGTCTT |
uc011jsq.2 | TAATTGAGAAGAGGCAGGAAATCAC | TTCAAGGTACAATCCTCCTTCTGG |
ENST00000456176 | AGCCAGACCTAGGCAGTATTTCAG | CCACAACAATTGAAACTGCAGTATG |
LncRNA00638 | GACCAGTGGAACTGATGGACACA | CTTGGGATTCCACAAATGACACT |
ENST00000562852 | CCTTAGAAGAAGAGGAGATTGGG | GGCAAGGTTGGTTTCATTCTG |
AK021458 | GCTCTGGGTACCAAGGACACA | CCACCCTTTCTGGTCATTTGC |
TCONS_00014557 | GTTGAGGGTGCAGTGAGTTGTG | AATGGGTGCTCTTGGAGAGTTT |
TCONS_00008000 | ACCTGTGAAGATGGAAGGAACC | TTCAAGGTACAATCCTCCTTCTGG |