Fig. 1

Derivation and action potential characterization of ventricular and atrial iPSC-derived cardiomyocytes. A Protocols for deriving ventricular and atrial cardiomyocytes from iPSCs, including three variations on atrial differentiation (standard, low growth factors (Low GF), and day 1 RA (D1RA)) applied to the base ventricular protocol. B Example action potential traces from two separate differentiations (1, 2) for each protocol; highlighting variability between differentiations. Time scale = 1 s. Summary of spontaneous action potential duration (APD) properties: C APD390, D APD90, E ratio of APD30 to APD90, F beat period. Each color represents a separate matched differentiation displayed as a violin plot, and individual violin plots are combined into superviolin plots (see methods for detailed explanation). Statistics performed with a mixed effects model. N/n = independent differentiations/technical repeats for ventricular (6/32), Atrial Standard (7/48), Atrial (Low GFs) (6/51), Atrial (D1RA) (11/85). ****p < 0.0001