Fig. 2

Multi-electrode array (MEA) characterization of spontaneous ventricular and atrial iPSC-derived cardiomyocyte field potentials. A The electrical properties of syncytium produced by each differentiation protocol were compared utilizing a MEA, with representative field potential traces from MEA recordings displayed. Short traces (4 s) and single FPD traces are shown. The red arrow indicates position of FPD measurement. Summary of MEA recordings: B beat period, C field potential duration, corrected for beat period using Fredericia’s formula, D spike amplitude, and E conduction velocity. Statistics performed with a mixed effects model. N/n = independent differentiations/technical repeats for ventricular (6/69), Atrial Standard (4/24), Atrial (Low GFs) (4/38), Atrial (D1RA) (10/107). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001