Fig. 4

Atrial (D1RA) protocol could be used on four additional iPSC lines without re-optimization. Four additional iPSC lines were differentiated without optimization and examined for their electrical properties. Example action potential traces are shown in (A(i–iv)) for lines 8C1, 473C1, 273C1, and VG1-C, respectively. Scale bars = 1 s. Summary of quantified action potential recordings, B beat period, C APD30, D, APD90, E APD30:90 ratio. Each cell line was monitored for electrical properties on a multi-electrode array with, F beat period, G corrected field potential duration, and H conduction velocity quantified. N/n = independent differentiations/technical repeats for 8C1 (4/19), 473C1 (1/3), 273C1 (1/9), VG1-C (3/10). Error bars are ± 1 SD