Fig. 4

Functional analysis of stemness subtypes and immunological analysis. A Violin diagram showing TMB differences between stemness subtypes, green indicates Stemness Subtype I, yellow indicates Stemness Subtype II. B Waterfall diagram showing the 10 genes with the highest mutation frequencies in Stemness Subtype I and Stemness Subtype II samples, different colors indicate different mutation types. The different colors indicate the different mutation types. C Copy number difference analysis of Stemness Subtype I and Stemness Subtype II, red indicates amplification and blue indicates deletion. gistic analysis assigns a G-score to each mutation, which indicates the magnitude of the mutation. D G-score differences between Stemness Subtype I and Stemness Subtype II were analyzed using box plots. E Differences in immune cells in different subgroups. F Comparison of differences in immune scores, stromal scores in different stemness subtypes by violin plots, ****Indicates P value < 0.0001. G Differences in immune subtype distribution in different stemness subtypes, sky blue indicates low immunity, brown indicates high immunity. H Analysis of differences in immune checkpoint genes. Red indicates Stemness Subtype I, green indicates Stemness Subtype II. I Gene mutation in different stemness subtypes, sky blue indicates no mutation in the sample, brown indicates mutation in the sample, * indicates P value < 0.05, ** indicates P value < 0.01, *** indicates P value < 0.001, ns indicates no statistical significance