Fig. 2
Profile of Basal MenSCs-derived EVs cargo proteome. A Characterization of the protein content of EV preparations by SDS-PAGE according to MISEV Guidelines [30]. Detection of proteins in category 1a (as tetraspanins CD63 and CD81), 2a (as TSG101, ALIX, and FLOT1), 2b (as GAPDH), and 4c (as CANX) are shown. A cell lysate, CL, (7.5 μg of total extract) was used as a control parallel to 4 × 109 particles isolated from the equally 1:1 pooled or the corresponding individual EV samples (n = 5). Molecular weights are indicated. Uncropped blots are presented in Additional file 2 and Additional file 3. GO enrichment of proteins identified in B-EVs. The most significant terms were clustered by the three GO subontologies: B Biological process (BP); C cellular component (CC); D molecular function (MF). E Reactome enrichment chart. The most significant processes are highlighted in blue and the least significant processes in red according to Benjamini-Hochberg-adjusted p values. Larger dots in the graphs indicate a greater number of proteins involved. Only the top 20 categories are shown. F Network graph obtained from Metascape composed of significantly enriched categories colored according to the functional cluster they belong to. Node size depends on the number of proteins annotated within the corresponding category. EVs, extracellular vesicles; MenSCs, menstrual blood-derived stromal cells; B-EVs, EVs released by basal MenSCs