Fig. 3

Proteomic alterations in EVs were obtained following different preconditioning of MenSCs. Proteomic data of different biogroups was filtered (detection in at least three donors) and comparatively analyzed. A Principal Component Analysis (PCA) showed a high level of clustering between biogroups. B Venn diagram depicting overlapping DAPs identified in the different EV samples. DAPs identified in B-EVs (red), PI-EVs (green), PHY-EVs (blue), and AH-EVs (yellow) are represented. C Volcano plots of differentially expressed proteins in PI-EVs (left), PHY-EVs (middle), and AH-EVs (right) vs. B-EVs. Values indicate the log2FC (X-axis) and –log10adjusted p value (Y-axis). Significantly (p < 0.01) increased (red dots, log2FC ≥ 1) and decreased (green dots, log2FC ≤ -1) proteins in the preconditioning vs. basal conditions are highlighted. Top-10 dysregulated proteins are depicted on the volcano plots, together with common EV markers. DAPs: differential abundant proteins; EVs, extracellular vesicles; MenSCs, menstrual blood-derived stromal cells; B-EVs, EVs released by basal MenSCs; PI-EVs, EVs released by pro-inflammatory primed MenSCs; PHY-EVs, EVs released by physioxia cultured MenSCs; AH-EVs, EVs released by acute hypoxia cultured MenSCs