Fig. 2
From: PAI-1 regulates AT2-mediated re-alveolarization and ion permeability
Fibrinolytic niche regulates the fate of AT2 cells in 3D organoids in vitro. a FACS analysis of AT1 (ICAM+) and AT2 (EpCAM+) cells harvested in organoids. b Cell count for AT1 and AT2 cells (N = 3 pairs of mice). c Representative immunofluorescence images of organoids at day 12. Organoids were labeled with pdpn (1:200) and sftpc (1:200) antibodies for AT1 and AT2 cells, respectively. Images of organoids were captured by Zeiss confocal microscope. Images were analyzed using ImageJ software (scale bar 100 µm). d Quantitative analysis of AT1–AT2 ratio in organoids between wt and Serpine1Tg mice (N = three technical replicates). Multiple unpaired t-test was used to compare AT1:AT2 ratio between wt and Serpine1Tg mice. Data expressed as Mean ± SEM. Unpaired t-test and two-tailed p < 0.05 were used to compare the means between wt and Serpine1Tg group