Fig. 3

Effect of elevated PAI-1 on re-alveolarization of AT2 cells. a Schematic representation of AT2 cells isolation, sorting, and culturing in parallel from wt and Serpine1^Tg mice. b FACS comparison of CD44⁺ AT2 cells between wt and Serpine1^Tg mice. c Yield of CD44⁺ AT2 cells in vivo between wt and Serpine1^Tg mice (N = 9 pairs of mice). d DIC images of AT2 organoids for wt and Serpine1^Tg mice. Scale bar, 100 μm. e Organoid number and f total surface area of organoids per transwell (n = 15 transwells per group). Data are represented as ± SEM, *p < 0.05 vs. wt by two-tailed Student’s t-test