Fig. 5

Inhibition of MAPK signalling pathway in Dex-induced muscle atrophy C2C12 cells. A A schematic diagram for in vitro cell experiment. (B-C) The establishment of Dex-induced C2C12 cells as a muscle atrophy in vitro model. n = 4 per group. B Immunofluorescence staining for myosin heavy chain and the quantification for myotube fusion index. C Real-time PCR analysis of muscle atrophy genes. D The myosin heavy chain staining and fusion index calculation of ADSCs-treated atrophy C2C12 cells with MAPK inhibitors (JNK, p38 and ERK1/2). n = 3 per group. E Real-time PCR analysis of muscle atrophy genes for samples treated with inhibitors. n = 6 per group. F, G ERK1/2 protein expression for in vitro (F) and in vivo (G) samples. Quantitative data are presented as mean ± SD. Statistical analysis are performed using one-way ANOVA test (A-B, D) and unpaired t-test (C), with significance set at P < 0.05 (*P < 0.05, **P < 0.01, ***P < 0.005, #P < 0.001)