Fig. 1

Analysis of human striatal grafts at short and long transplantation times in standard housing. A Differences in graft volume between 2 and 6MPT (unpaired t test; p < 0.05; N = 6 animals 2MPT; 5 6MPT SE). B Comparison of graft cell density at 2MPT and 6MPT (unpaired t test p < 0.001; N = 6 animals 2MPT; 4 6MPT SE). C Images depict the grafted hemisphere at 2MPT and 6MPT showing graft size and position (dotted lines) at 2MPT or 6MPT. D Proportions of HuNu⁺ cells expressing the proliferative marker Ki67 (unpaired t test, p < 0.01; N = 6 2MPT; 4 6MPT), or the immature marker NESTIN (unpaired t test, p < 0.01; N = 6 2MPT; 4 6MPT), or the astrocyte marker GFAP (unpaired t test, p < 0.01; N = 6 2MPT, 3 6MPT) at 2 or 6 MPT in HuNu⁺ cells (N = 6 animals 2MPT; 5 6MPT SE). E Proportions of striatal marker expression in HuNu⁺ cells at 2 and 6 MPT (unpaired t test, 2MPT vs. 6MPT SE; CTIP2, p > 0.05 ns; DARPP32, p < 0.001; CTIP2/DARPP32, p < 0.001; GABA, p > 0.05 ns; N = 6 animals 2MPT; 5 6MPT SE). F and F′ Progressive maturation of hESC-derived striatal progenitors (HuNu⁺) into MSN neurons expressing CTIP2 (in red) and DARPP32 (in white). Arrowheads indicate some HuNu⁺/CTIP2⁺ neurons. G Proportions of interneuron markers expressed by HuNu⁺ neurons at 2 and 6 MPT (unpaired t tests, p < 0.001; CB and CR, N = 6 2MPT; 4 6MPT SE; TH, N = 4 2MPT, 3 6MPT). H and H′ Representative graft images showing a fraction of grafted cells (in green) positive for calbindin (CB, in red) at 2 and 6 MPT. Data are represented as mean ± SEM. Scale bars: 1 mm (C); 50 μm (F–H′)