Fig. 3

Muse improve survival by maintaining the intestinal integrity in GIS mouse model. A Representative images of immunofluorescence showing Lysozyme-positive cells (red) in the small intestine of non-treated or Muse-treated mice at 7 days after irradiation. Integrated GFP⁺ Muse (green) are identified with white arrows. Nuclei were counterstained with DAPI (blue). B Kaplan–Meier survival analysis (left) and weight loss changes (right) for 29 days of 18 Gy abdominal exposed mice receiving or not a 50,000 MSC or 50,000 Muse-treatment 4 h after irradiation. Statistical difference of survival between groups was determined by Log-rank (Mantel-Cox) test, with *p ≤ 0.05 considered as significant. Weight data are represented with means ± SEM. C Kaplan–Meier survival analysis (left) and weight loss changes (right) for 29 days of 18 Gy abdominal exposed mice receiving or not a 50,000 Muse-treatment 4 h, 24 h or 5 days after irradiation. Statistical difference of survival between groups was determined by Log-rank (Mantel-Cox) test, with *p ≤ 0.05 considered as significant. Weight data are represented with means ± SEM. D Illustration (left), length (middle) and weight (right) of the small intestine of non-treated or Muse-treated mice 7 days after irradiation, compared to non-irradiated (NIR) control mice. Data are represented with means ± SEM, *p < 0.05; ns: not significant (two-tailed Mann–Whitney U test). E Representative HE staining of small intestine of non-treated or Muse-treated mice at 1, 3.5 and 7 days after irradiation, compared to non-irradiated control mice. F Histogram plots showing the percentage of surviving clonogenic crypts in small intestine of non-treated or Muse-treated mice at 3.5 days after irradiation, compared to non-irradiated control mice. Data are represented with means ± SEM, *p < 0.05; ****p < 0.001 (two-tailed Mann–Whitney U test)