Fig. 4

Muse are required for intestinal epithelium regeneration. A Intestinal permeability illustrated by histogram plots showing 4-kDa FITC-Dextran levels measured in the plasma of non-treated or Muse-treated mice at 7 days after irradiation, compared to non-irradiated control mice. Data are represented with means ± SEM, *p < 0.05; ****p < 0.001 (two-tailed Mann–Whitney U test). B Representative immunohistochemical images showing the expression of ZO-1 protein (brown) in ileum sections of non-treated or Muse-treated mice at 7 days after irradiation, compared to non-irradiated control mice. Nuclei were counterstained with hematoxylin (blue). C Representative immunofluorescent images showing EpCAM-positive cells (red) in the small intestine of non-treated or Muse-treated mice at 7 days after irradiation, compared to non-irradiated control mice. Nuclei were counterstained with DAPI (blue). D Representative immunofluorescent images showing CD24-positive cells (green) and Ki67-positive cells (red) in the small intestine of non-treated or Muse-treated mice at 7 days after irradiation, compared to non-irradiated control mice. Nuclei were counterstained with DAPI (blue)