Fig. 5

Monocyte/M2-Macrophage recruitment is induced by Muse treatment. A Histogram plots showing MCP-1 secretion level in supernatant of 7 h-cultured terminal ileum excised from non-treated or Muse-treated at 1 or 7 days after irradiation, compared to non-irradiated control mice. Data are represented with means ± SEM, *p < 0.05; **p < 0.01 (two-tailed Mann–Whitney U test). B Representative flow cytometry gating strategy for analysis of monocyte subset in lamina propria isolated from small intestine of non-treated (top) or Muse-treated (bottom) mice at 1 day after irradiation. Histograms plots showing the proportion of M1-like monocytes (Ly6C^hi) among the alive CD45⁺ population from the lamina propria (left) and lamina epithelialis (right) fractions of non-treated or Muse-treated mice at 1 day after irradiation, compared to non-irradiated control mice (bottom). Data are represented with means ± SEM, *p < 0.05 (two-tailed Mann–Whitney U test). C Representative immunofluorescence images showing CD68-positive (green) and CD206-positive (red) macrophages in the small intestine of non-treated or Muse-treated mice at 7 days after irradiation, compared to non-irradiated control mice. Nuclei were counterstained with DAPI (blue)