Fig. 6

Muse enhance Paneth cell proliferation and IL-6/Stat3 signaling pathway. A Histogram plots showing IL-6 level in supernatant of 7-h-cultured intestine excised from non-treated or Muse-treated mice 1 or 7 days after irradiation, compared to non-irradiated mice. Data are represented with means ± SEM, *p < 0.05 (two-tailed Mann–Whitney U test). B Representative immunofluorescence images showing Lysozyme-positive cells (red) in the ileum of non-treated or Muse-treated mice 1 day after irradiation, compared to non-irradiated mice. Nuclei were counterstained with DAPI (blue) (top). Histogram plots showing Paneth cell number per crypts (bottom). Data are represented with means ± SEM, *p < 0.05; **p < 0.01 (two-tailed Mann–Whitney U test). C Histograms plots showing total cell number in the lamina epithelialis fraction (left) and proportion of Paneth cells (CD24^hiCD166^med/+) among CD45^neg population (right) in non-treated or Muse-treated mice 1 day after irradiation. Data are represented with means ± SEM, **p < 0.01 (two-tailed Mann–Whitney U test). D Representative cropping western blot showing protein level of cleaved caspase-3 in Paneth cells isolated from two non-treated and two Muse-treated mice 1 day after irradiation (left). GAPDH was used as internal control. Full length blots are presented in Additional file 1: Fig. S4A. Quantitative analysis of cleaved caspase 3 protein level normalized to GAPDH (right). Data are represented with means ± SEM. E Representative immunofluorescence images showing Lysozyme-positive cells (green) and Ki67-positive cells (red) in ileum of non-treated or Muse-treated mice 1 day after irradiation, compared to non-irradiated mice. Nuclei were counterstained with DAPI (blue). F Representative cropping western blot showing protein levels of p-Stat3 and Stat3 in Paneth cells isolated from two non-treated and two Muse-treated mice 1 day after irradiation. GAPDH was used as internal control (left). Full length blots are presented in Additional file 1: Fig. S4B. Quantitative analysis of the p-Stat3/Stat3 ratio after normalization to GAPDH (right). Data are represented with means ± SEM. *p < 0.05 (two-tailed Mann–Whitney U test)