Fig. 1

NG2⁺ cell-originated muscle fibers in a muscle-type dependent manner. A NG2⁺ PC-specific lineage tracing was performed using NG2-CreERT/Rosa-tdTomato mice. Immunostaining short axis view of lower leg muscle (gastrocnemius and soleus) demonstrates the distribution of each type of muscular fiber, types I, IIA, and IIB. A dashed line square area indicates the soleus. Scale bar = 500 µm. B High-power immunostaining view of the soleus area. Scale bar = 200 µm. C The representative fluorescence view of tdTomato⁺ myofibers within the soleus and gastrocnemius is shown at the indicated time labeling of NG2⁺ PCs. D The time course of the proportion of labeled myofibers to total myofibers is shown. The values are presented as the means ± standard error of the mean (SEM); n = 3–4