Fig. 3

In vitro myogenic potency of NG2⁺ PCs attached around myofibers. A Myofibers isolated from the soleus of NG2-CreERT/Rosa-tdTomato mice, which were incubated in DMEM-containing hydroxy tamoxifen (Tam) for 3 days to label NG2⁺ PCs. After 6 days of differentiation induction, myogenesis was determined by immunostaining with myosin heavy chain (MyHC). Nuclei are counterstained by DAPI. Scale bars = 100 µm. B Gene expression of myogenesis-related genes in each sample, determined by quantitative RT-PCR analysis. Open bars = differentiation induction (–), and closed bars = differentiation induction ( +). The values are presented as mean ± SEM (n = 4). *P < 0.05, **P < 0.01