Fig. 7

Muscle fiber types and gene expression profiles in soleus after NG2⁺ PC-deletion. A At 4 months after PC deletion, the muscle types of each myofiber in the soleus were determined by immunostaining, and the proportion of each myofiber type was calculated (n = 4). Scale bar = 50 µm. B functional vasculature within muscles was estimated as the area of rhodamine-lectin-stained microvessels per observation area of soleus. Expression of myogenic stem cell marker genes (C) and myogenesis-related genes within the soleus muscle (D) estimated by quantitative RT-PCR. Closed bars = PC deletion (PC-del), open bars = control (Ct). The values are presented as the means ± SEM (n = 3–5); *P < 0.05, **P < 0.01, ns = not significant