NG2-positive pericytes regulate homeostatic maintenance of slow-type skeletal muscle with rapid myonuclear turnover

Tatsukawa, Takamitsu; Kano, Kohei; Nakajima, Kei-ichi; Yazawa, Takashi; Eguchi, Ryoji; Kabara, Maki; Horiuchi, Kiwamu; Hayasaka, Taiki; Matsuo, Risa; Hasebe, Naoyuki; Azuma, Nobuyoshi; Kawabe, Jun-ichi

doi:10.1186/s13287-023-03433-1

Stem Cell Research & Therapy

Table 1 Comprehensive gene expression profile in response to PC deletion

From: NG2-positive pericytes regulate homeostatic maintenance of slow-type skeletal muscle with rapid myonuclear turnover

Group	Symbol	Gene description	Normalized expression		Ratio (fold)	Up/down
Group	Symbol	Gene description	Control	PC-deletion	Ratio (fold)	Up	Down
Cell marker	CSPG4/NG2	Chondroitin sulfate proteoglycan 4	N.D	15	15.23	***
	PDGFRβ	Platelet-derived growth factor receptor, beta polypeptide	101	152	1.51
	Pax7	Paired box 7	N.D	N.D	1.00
	PDGFRα	Platelet-derived growth factor receptor, alpha polypeptide	310	410	1.32
	PECAM1	Platelet/endothelial cell adhesion molecule 1	N.D	N.D	0.65
	ACTA1	Actin, alpha 1, skeletal muscle	36,501	13,449	0.37		*
	ACTA2	Actin, alpha 2, smooth muscle, aorta	387	628	1.63
Myogenic marker	Myf5	Myogenic factor 5	N.D	13	12.59	***
	Myod1	Myogenic differentiation 1	35	50	1.43
	Myogenin	Myogenin	21	403	19.06	***
	Myf6	Myogenic factor 6	1168	1303	1.12
	Myh2	Myosin, heavy polypeptide 2, skeletal muscle, adult	26,914	10,276	0.38		*
	Myh3	Myosin, heavy polypeptide 3, skeletal muscle, embryonic	97	1167	12.07	***
	Myh4	Myosin, heavy polypeptide 4	1515	2887	1.91
	Myh7	Myosin, heavy polypeptide 7	265	78	0.30		*
Slow myofiber maintenance	NFATc1	Nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1	37	26	0.69
	PPARGC1a	Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha	90	58	0.64
	MEF2a	Myocyte enhancer factor 2A	82	77	0.95
	MEF2c	Myocyte enhancer factor 2C	883	579	0.66
	MEF2d	Myocyte enhancer factor 2D	256	121	0.47		*
	FOXO3	Forkhead box O3	152	239	1.57
Hypoxic	HIF1α	Hypoxia inducible factor 1, alpha subunit	78	163	2.11	*
	BNIP3	BCL2/adenovirus E1B interacting protein 3	4452	2064	0.46		*
	PDK1	Pyruvate dehydrogenase kinase, isoenzyme 1	207	125	0.60
	Slc2a1/GLUT1	Solute carrier family 2 (facilitated glucose transporter), member 1	57	62	1.10
Proliferation/Growth factor	PDGFα	Platelet-derived growth factor, alpha	23	31	1.34
	PDGFβ	Platelet-derived growth factor, B polypeptide	N.D	11	11.45	***
	VEGFα	Vascular endothelial growth factor A	114	63	0.55
	VEGFβ	Vascular endothelial growth factor B	1362	474	0.35		*
	Egf	Epidermal growth factor	87	119	1.37
	Fgf1	Fibroblast growth factor 1	221	114	0.51
Apoptosis	Casp2	Caspase 2	10	26	2.47	*
	Casp3	Caspase 3	21	129	6.07	**
	Casp6	Caspase 6	30	34	1.12
	Casp7	Caspase 7	8	28	3.67	*
	Casp9	Caspase 9	31	12	0.38		*
	Bax	BCL2-associated X protein	78	114	1.46

After induction of NG2⁺ PC deletion for 1 month, gene expression within the soleus of PC-deletion and control mice was estimated by microarray analysis. The signal for each gene was normalized using the global normalization method. The definition of a significant difference was more or less than twice (up/down * 2, ** 4, *** 8) the difference in the log2 ratio among genes with a fluorescence value of > 100 (boldface). ND Not detected

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ISSN: 1757-6512

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