Fig. 4

Transgenic hRPE survive and function long-term in immunodeficient mice. A Bioluminescent images of NSG mice treated with subretinal injections of FS-Luc hRPE. B OTX2 and human nuclear antigen (HuNu) co-staining of NSG retinas treated with subretinal injections of either FS-Luc hRPE or FS-CX3-9 hRPE at 2 weeks post-injection (n = 1 eye) and 4 months post-injection (n = 2–4 eyes). C Co-staining of FS-CX3-9 hRPE retinas for FLAG epitope (to detect exogenous sCX3CL1) and HuNu (n = 2 eyes). D Quantification of OTX2⁺/HuNu⁺ cells in the subretinal space of NSG mice at 4 months post-injection across a 200 µm distance (n = 2–4 eyes, each consisting of 3 technical replicates of tissue sections analyzed). Scale is 50 µm. Student’s t-test was performed to assess statistical significance. ONL, outer nuclear layer; SRS, subretinal space; WPI, weeks post-injection; MPI, months post-injection; ns, not significant