Fig. 5

Donor hRPE survive short-term in the subretinal space of the degenerating retina. A Experimental timeline: Postnatal (PN) 14 Rd10 mice were treated with subretinal injections of FS-Luc or FS-CX3 hRPE cells and sacrificed at PN28 or PN35 for analysis. B Bioluminescence imaging of rd10 mice treated with subretinal injections of Luc hRPE. C Immunostaining of rd10 retinas for OTX2 and human nuclear antigen (HuNu) (n = 3–5 eyes). D Immunostaining of rd10 retinas for FLAG epitope (to detect exogenous sCX3CL1) and HuNu (n = 3 eyes). E Quantification of OTX2⁺/HuNu⁺ cells in the subretinal space of rd10 mice on PN28 across a 200 µm distance (n = 3–5 eyes, each consisting of 3 technical replicates of tissue sections analyzed). Scale is 50 µm. Each data point represents one biological replicate consisting of three technical replicates. Student’s t-test was performed to assess statistical significance. ONL, outer nuclear layer; SRS, subretinal space; WPI, weeks post-injection; ns, not significant