Fig. 6

Soluble CX3CL1-expressing hRPE preserve photoreceptors in the rd10 mouse. Rd10 mice were treated on PN14 with subretinal injections of FS-Luc or FS-CX3-9 hRPE in HAMC, or HAMC alone (sham). Mice were sacrificed on PN28 for analysis. A Schematic for tissue analysis. Proximal region is indicated by presence of cell graft or evidence of retinal detachment. Distal region is on the opposite side of the cell graft of the same tissue section, equidistant from the optic nerve. B Retinas immunostained for rhodopsin. The mCherry fluorescent reporter is expressed by injected cells. (n = 3–7 eyes) Scale is 50 µm. C Quantification of rows of nuclei. (n = 3 eyes for sham and n = 5 eyes for hRPE treatment groups, each consisting of 3 technical replicates or sections analyzed). Error bars represent SEM. One-way ANOVA and Tukey’s post-hoc analysis performed for statistical analysis. All comparisons are not significant unless otherwise stated. ***p < 0.001, ****p < 0.0001. ONL, outer nuclear layer; SRS, subretinal space