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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Single-cell RNA sequencing of human non-hematopoietic bone marrow cells reveals a unique set of inter-species conserved biomarkers for native mesenchymal stromal cells

Fig. 1

Human adult bone marrow (BM) single-cell sequencing analysis. A Fourteen CD45-/low, CD235- BM sorting cell clusters were obtained after analysis. UMAP visualization of 1677 cells (n = 5), annotated post hoc and colored by Louvain clustering. B Non-hematopoietic cluster identification and depiction by UMAP and violin visualizations, annotated post hoc with their most representative marker: CXCL12, PDGFRA and LEPR for mesenchymal stem/stromal cells (MSCs), RGS5, CD146 and PDGFRB for mural cells (MCs), and claudin 5 (CLDN5), FLT1 and CD105 for endothelial cells (ECs). C Top genes expressed by MSCs (10), MCs (11) and ECs (12) after analysis of differentially expressed genes (DEGs). Data are depicted as genes significantly more expressed compared to the other clusters of cells. D Top 60 DEGs (columns) for each non-hematopoietic cell subsets (rows labeled on top of diagram) ranked after dendrogram analysis. E Gene Ontology (GO) terms of biological processes for DEGs for each non-hematopoietic cluster. Analysis calculated from Enrichr and ranked by combined score [75]

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Stem Cell Research & Therapy

ISSN: 1757-6512