Fig. 2

Human BM-MSC characterizations. A Representative CellphoneDB analysis matrix plot of ligand-receptor expression between each cluster and between MSCs, MCs, and ECs. The heatmap depicts the number of significant interactions between the different types of cells. Below, the interactions are defined by the type of receptor-ligand potentially active between MSCs and all of other clusters. B Heatmap representation of the genes expressed by native human BM-MSCs directly sorted (CD271 + /CD200 + /CD45−) and statistically compared to the CD271 + /CD200−/CD45− BM population. Study performed by Affymetrix mircoarrays. mRNA expression of representative markers of MSCs obtained from microarrays (n = 6; *p < 0.05; **p < 0.001; ***p < 0.0001). C Gene set enrichment analysis (GSEA) plot showing similarities between human BM CD271 + sorting cells from Li et al. study [76] and our study described above. Results were statistically significant according to normalized enrichment score (NES), false discovery rate (FDR) and p value depicted on the panel. D Distribution of the most correlated genes with the MSC subset from 193 enriched genes identified by GSEA analysis. Their expression was compared to that found for MCs and ECs