Fig. 2
Characterization of EVs isolated from MSCs cultured under normoxic (18.4% O2) and physioxic (3% O2) conditions. A Size distribution measured by SP-IRIS of particles in EV fractions isolated from normoxic and physioxic MSC cultures. B Number of CD63, CD81, and CD9 expressing particles isolated from normoxic and physioxic MSC cultures as quantified by SP-IRIS (N = 3). Total concentration is expressed as particles/ml normalized to CM and EV yield is expressed as particles/cell. C Biomolecule concentrations for EV fractions from normoxic and physioxic cultures as measured using Luminex (N = 3). D % of syntenin-1 expressing particles as measured by % of total CD63 expressing particles (N = 3). E % of GRP94 expressing particles as measured by % of total CD63 expressing particles (N = 3). F Representative TEM images of normoxic and physioxic samples taken at 50,000× and 60 kV, with arrows indicating typical EV cup-shape morphology. Scale bar = 100 nm. *P < 0.05, **P < 0.01, bars represent mean and SD