Fig. 3
Effect of EVs isolated from MSCs cultured in normoxic (18.4% O2) and physioxic (3% O2) conditions on CMEC proliferation. A Number of cells per well as measured by a CyQUANT Proliferation assay for CMECs under normoxic and physioxic conditions after 48 h. N = 3, *P < 0.05, ***P < 0.001, bars represent mean and SD. B 10× phase contrast images of CMECs after 48 h of culture with treatments in EBM-2 (negative control), EGM-2 (positive control), and EBM-2 or EGM-2 supplemented with EVs at 20× original medium concentrations from normoxic and physioxic MSC cultures. Scale bar = 100 μm