Table 1 Human-specific primers used for RT-qPCR (F: forward; R: reverse)
Gene | Primer Sequence (5′-3′) | Origin |
|---|---|---|
18S | F: TGG TCG CTC GCT CCT CTC C R: CGC CTG CTG CCT TCC TTG G | NR_003286 |
ANG1 | F: CCT GAT CTT ACA CGG TGC R: GCT TTC ATA ATC GCT TCT | NM_001314051 |
ANG2 | F: ACG GAC CAA AGC AAG ACC R: GGT TGT GAC AGC AGC GTC | XM_017013318 |
BCL2 | F: GAT GAC TGA GTA CCT GAA CC R: AGT TCC ACA AAG GCA TCC | EU287875 |
FGF2 | F: CGC GGT TGC AAC GGG AT R: GGG TTC ACG GAT GGT TGT CT | NM_27968 |
CLDN5 | F: TTT CCC TAA CTT CAG CTG CC R: CCC TCT TTG AAG GTT CGG G | NM_001130861 |
HIF1 | F: CCA GTT ACG TTC CTT CGA TCA GT R: TTT GAG GAC TTG CGC TTT CA | NM_001243084 |
HIF2 | F: GGT GGC AGA ACT TGA AGG GTT A R: GGG CAA CAC ACA CAG GAA AT | NM_001430 |
HMOX1 | F: ATG ACA CCA AGG ACC AGA GC R: GTG TAA GGA CCC ATC GGA GA | NM_002133 |
IL6 | F: TCA ATA TTA GAG TCT CAA CCC CCA R: TTC TCT TTC GTT CCC GGT GG | NM_000600 |
MCP1 | F: GCA ATC AAT GCC CCA GTC AC R: TCT TTG GGA CAC TTG CTG CT | S71513 |
OCLN | F: GCA AAG TGA ATG ACA AGC GG R: CAC AGG CGA AGT TAA TGG AAG | NM_002538 |
SDF1 | F: GGA CTT TCC GCT AGA CCC AC R: GCC CGA TCC CAG ATC AAT GT | NM_199168 |
TGFB1 | F: GGG GAA ATT GAG GGC TTT CG R: CCA GGA CCT TGC TGT ACT GC | NM_000660 |
VEGF | F: ACG GTC CCT CTT GGA ATT GG R: GGC CGC GGT GTG TCT A | M32977 |
TJP1 | F: TGC TGA GTC CTT TGG TGA TG R: AAT TTG GAT CTC CGG GAA GAC | NM_003257 |