Fig. 3

miR-143-3p mediates ASC senescence and cellular functions. a β-gal Staining analysis of senescent cells among ASCs treated with miR-143-3p mimic, mimic-NC, miR-143-3p inhibitor, or inhibitor-NC. Scale bar = 100 µm. b Representative images of migrated cells and quantitative analysis of ASCs that received the above treatment. Scale bar = 100 µm. c Representative images and quantitative analysis of EdU-stained cells (green) in ASCs that received the treatment; nuclei were stained blue. Scale bar = 100 µm. d Images of tube formation and analysis of HUVECs treated with CM from ASCs treated with miR-143-3p mimic, mimic-NC, miR-143-3p inhibitor, or inhibitor-NC. Scale bar = 100 µm. e Images and analysis of migrated fibroblasts treated with CM as described above. Scale bar = 200 µm. f Western blotting analysis of the expression levels of p21, FN1, and CCNA1 in ASCs transfected with miR-143-3p mimic, mimic-NC, miR-143-3p inhibitor, or inhibitor-NC. g Qualified data shown in F. Data are shown as the mean ± SEM. All experiments were performed in triplicate. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 versus mimic-NC group, #p < 0.05, ##p < 0.01, ###p < 0.001 versus inhibitor-NC group